Abstract | OBJECTIVE: METHODS: RESULTS: ANPC and CE groups have 29 and 39 DEMs compared to the CTRL group, respectively. Based on these 25 DEMs, interactions between miRNA and mRNA were screened, and 174 potential target genes were identified. We performed gene ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis on these 174 target genes, and the results revealed that the three pathways with the highest enrichment were the PI3K-Akt signaling pathway, FoxO signaling pathway, and Focal adhesion. The interaction analysis of PPI and miRNA-hub gene networks revealed that interleukin 6 (IL-6) was regulated by miR-146a-5p and miR-149-5p, while IL-10 was regulated by miR-29b-3p and miR-29c-3. Using reverse transcription polymerase chain reaction, we found that the miRNAs regulating IL-6 and IL-10 were significantly upregulated in the ANPC group, and there are three pathways involved in that process: Pathways of PI3K-Akt signaling, FoxO signaling, and Focal adhesion. IL-6 and IL-10 play an important role in cellular pyroptosis and apoptosis. Therefore, the aforementioned results provide significant reference value for elucidating the mechanism of cellular pyroptosis and apoptosis in echinococcosis-induced anaphylaxis, and for formulating tissue and organ protection strategies for patients with cystic echinococcosis when anaphylaxis is triggered by hydatid cyst rupture.
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Authors | Chun-Sheng Wang, Tao Yu, Xilizhati Kulaixi, Jing-Ru Zhou, Xianyidan Abulajiang, Jia-Ling Wang, Si-Jia Wang, Jian-Rong Ye |
Journal | Immunity, inflammation and disease
(Immun Inflamm Dis)
Vol. 11
Issue 8
Pg. e961
(08 2023)
ISSN: 2050-4527 [Electronic] England |
PMID | 37647453
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2023 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd. |
Chemical References |
- Interleukin-6
- Interleukin-10
- Phosphatidylinositol 3-Kinases
- Proto-Oncogene Proteins c-akt
- MicroRNAs
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Topics |
- Animals
- Mice
- Echinococcus granulosus
(genetics)
- Interleukin-6
(genetics)
- Interleukin-10
(genetics)
- Anaphylaxis
(genetics)
- Phosphatidylinositol 3-Kinases
- Proto-Oncogene Proteins c-akt
- Echinococcosis
- Mice, Inbred BALB C
- MicroRNAs
(genetics)
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