Antitumor and antibacterial activity of metabolites of endophytic Colletotrichum siamense isolated from coffee (Coffea arabica L. cv IAPAR-59).

Abstract

Endophytic fungi produce a range of known metabolites and several others, not yet explored, which present important biological activities from the pharmaceutical and industrial perspective. Several studies have reported the diversity of endophytes in Coffea arabica plants, although few have been described in organic cultures. In the current paper, we describe the chemical profile of specialized metabolites in the ethyl acetate phase in a strain of the endophytic fungus Colletotrichum siamense associated with coffee (Coffea arabica L.) (Rubiaceae) and its potential against tumor cells and bacteria of medical and food importance. Cytotoxicity assays in tumor cells MCF-7 and HepG2/C3A were performed by MTT and microdilution in broth to evaluate the antibacterial action of metabolic extract. The antiproliferative assay showed promising results after 24 h of treatment, with 50% injunction concentrations for the two cell types. UHPLC-MS/MS analyses with an electrospray ionization source were used to analyze the extracts and identify compounds of species Colletotrichum siamense, which is still little explored as a source of active metabolites. Many of these compounds observed in the endophytic need to be chemically synthesized in industry, at high costs, while production by the fungus becomes a chemically and economically more viable alternative. Pyrocatechol, gentisyl alcohol, and alpha-linolenic acid, associated with different mechanisms of action against tumor cells, were detected among the main compounds. The extract of the endophytic fungus Colletotrichum siamense presented several compounds with pharmacological potential and antibacterial activity, corroborating its potential in biotechnological applications.

• Authors: Bruno César do Espírito Santo, João Arthur Dos Santos Oliveira, Marcos Alessandro Dos Santos Ribeiro, Rodrigo Pawloski Schoffen, Andressa Domingos Polli, Julio Cesar Polonio, Angela Aparecida da Silva, Benício Alves de Abreu Filho, Michele Cristina Heck, Eduardo César Meurer, Paola Pereira Constantin, Marcos Pileggi, Veronica Elisa Pimenta Vicentini, Halison Correia Golias, João Alencar Pamphile
• Journal: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (Braz J Microbiol) Vol. 54 Issue 4 Pg. 2651-2661 (Dec 2023) ISSN: 1678-4405 [Electronic] Brazil
• PMID: 37642890 (Publication Type: Journal Article)
• Copyright: © 2023. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.

Chemical References

• Coffee
• Anti-Bacterial Agents
• Plant Extracts

Topics

• Coffea (microbiology)
• Coffee (metabolism)
• Tandem Mass Spectrometry
• Colletotrichum
• Anti-Bacterial Agents (pharmacology, metabolism)
• Plant Extracts (pharmacology, metabolism)
• Endophytes