Alterations of the salivary microbiota in gastroesophageal reflux disease.

Abstract

OBJECTIVES:

Gastroesophageal reflux disease (GERD) is among the most prevalent gastrointestinal disorders. The oral microbiota plays an important role in human health and may be altered by the presence of GERD. Here, we aimed to investigate the alterations of salivary microbiota in GERD patients.

METHODS:

We collected clinical information and salivary samples from 60 individuals. All participants underwent combined pH/impedance monitoring measurement and submitted samples for salivary microbiota sequencing. According to acid exposure time and DeMeester score, participants were divided into two groups: GERD + (Group G) and GERD - (Group C).

RESULTS:

There was no significant difference in alpha diversity between study groups. Regarding beta diversity, principal coordinate analysis plots indicated that the microbiota composition data of the participants were grouped within partial overlapping clusters. The statistical analysis of the distance matrices was performed using the Adonis test (p = 0.017). Based on linear discriminant analysis effect size, the relative abundances of the phylum Bacteroidetes, class Bacteroidia, order Bacteroidales, family Prevotellaceae, and genus unidentified_Prevotellaceae were enriched in Group G. Compared with Group C, the phylum Actinobacteria, classes unidentified_Actinobacteria and Bacilli, orders Micrococcales and Lactobacillales, families Micrococcaceae and Streptococcaceae, and genuses Rothia and Streptococcus were decreased in Group G. At the genus level, the abundances of Streptococcus and Rothia were negatively correlated with DeMeester score and acid exposure time.

CONCLUSIONS:

This study revealed alterations of the salivary microbiota in GERD patients, suggesting that acid reflux changes the oral ecosystem.

• Authors: Jun Qian, Meilin Yang, Duiyue Xu, Gaosong Zhang, Youhong Cai, Bin Yang, Xiying Wang, Yanbo Yu
• Journal: Journal of oral biosciences (J Oral Biosci) Vol. 65 Issue 4 Pg. 280-286 (12 2023) ISSN: 1880-3865 [Electronic] Netherlands
• PMID: 37595742 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2023 Japanese Association for Oral Biology. Published by Elsevier B.V. All rights reserved.

Topics

• Humans
• Gastroesophageal Reflux
• Microbiota
• Bacteria