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A highly sensitive and robust electrochemical biosensor for microRNA detection based on PNA-DNA hetero-three-way junction formation and target-recycling catalytic hairpin assembly amplification.

Abstract
Robust and sensitive methods for the detection of microRNAs (miRNAs) are crucial in the clinical diagnosis of cancers. In this study, a novel electrochemical biosensor with high sensitivity for miRNA-21 detection is developed, which relies on the formation of a peptide nucleic acid (PNA)-DNA hetero-three-way junction (H3WJ) and target-recycling catalytic hairpin assembly (CHA) amplification. The electroneutral PNA probes are initially immobilized onto a gold electrode to construct the sensor. Upon introduction of miRNA-21, target-recycling CHA is initiated, resulting in abundant double-stranded CHA products. Subsequently, association between the PNA probes and these products leads to the formation of PNA-DNA H3WJs. Consequently, the electrode surface is densely populated with numerous electroactive Ferrocene (Fc) groups, resulting in a significantly amplified current response for highly sensitive detection of miRNA-21 at concentrations as low as 0.15 fM. This approach demonstrates remarkable specificity towards target miRNAs and can be utilized for quantitative monitoring of miRNA-21 expression in human cancer cells. More importantly, the sensor exhibits exceptional stability and shows a significant reduction in background noise during miRNA detection, making this method a highly promising sensing platform for monitoring various miRNA biomarkers to facilitate the diagnosis of diverse cancers.
AuthorsXiaomeng Yu, Shuyu Ding, Yang Zhao, Mengjia Xu, Zimiao Wu, Chao Zhao
JournalTalanta (Talanta) Vol. 266 Issue Pt 1 Pg. 125020 (Jan 01 2024) ISSN: 1873-3573 [Electronic] Netherlands
PMID37541007 (Publication Type: Journal Article)
CopyrightCopyright © 2023 Elsevier B.V. All rights reserved.
Chemical References
  • MicroRNAs
  • Peptide Nucleic Acids
  • DNA
Topics
  • Humans
  • MicroRNAs (genetics)
  • Peptide Nucleic Acids
  • Biosensing Techniques (methods)
  • DNA
  • DNA Replication
  • Electrochemical Techniques
  • Limit of Detection

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