A Novel Heat Shock Protein 70-Based Vaccine Prepared from DC Tumor Fusion Cells: An Update.

Abstract

We have developed an enhanced molecular chaperone-based vaccine through rapid isolation of Hsp70 peptide complexes after the fusion of tumor and dendritic cells (Hsp70.PC-F). In this approach, the tumor antigens are introduced into the antigen-processing machinery of dendritic cells through the cell fusion process, and thus we can obtain antigenic tumor peptides or their intermediates that have been processed by dendritic cells. Our results show that Hsp70.PC-F has increased immunogenicity compared to preparations from tumor cells alone and therefore constitutes an improved formulation of the chaperone protein-based tumor vaccine.

• Authors: Desheng Weng, Stuart K Calderwood, Jianlin Gong
• Journal: Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol) Vol. 2693 Pg. 209-219 ( 2023) ISSN: 1940-6029 [Electronic] United States
• PMID: 37540437 (Publication Type: Journal Article)
• Copyright: © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Chemical References

• HSP70 Heat-Shock Proteins
• Antigens, Neoplasm
• Molecular Chaperones
• Peptides
• Cancer Vaccines
• Vaccines, Synthetic

Topics

• Humans
• HSP70 Heat-Shock Proteins
• Dendritic Cells
• Neoplasms (metabolism)
• Antigens, Neoplasm
• Molecular Chaperones (metabolism)
• Peptides (metabolism)
• Cancer Vaccines
• Vaccines, Synthetic
• T-Lymphocytes, Cytotoxic