Abstract | Research background: Ficus deltoidea (mistletoe fig) is a shrub well known among locals in Malaysia primarily for its treatment of toothaches, colds and wounds. The aim of this study is to determine the potential of leaves, sourced from three different varieties of F. deltoidea, to exhibit antioxidant activity, a reduction of lipid concentration, and protein expression in steatosis-induced liver cell lines. Experimental approach: The leaves of three F. deltoidea varieties, namely Ficus deltoidea var. angustifolia, Ficus deltoidea var. trengganuensis and Ficus deltoidea var. kunstleri, were subjected to water extraction. The resulting crude extracts were fractionated using water and ethyl acetate. Palmitic acid was used to induce lipid accumulation (steatosis) in human liver (WRL68) cells, before all the samples were tested for their lipid-reducing activity. Several proteomic approaches were incorporated. The changes in protein expression were determined using 2-dimensional gel electrophoresis separation, whereas identification of our protein spots of interest was carried out via matrix-assisted laser desorption/ionization time-of-flight. Results and conclusions: Novelty and scientific contribution: F. deltoidea pretreatment was shown to reduce lipid accumulation, thus improving the redox status and protein homeostasis. This suggests the role of F. deltoidea as a preventive mechanism in non- alcohol fatty liver disease.
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Authors | Noor Nazirahanie Abrahim, Norhaniza Aminudin, Puteri Shafinaz Abdul-Rahman |
Journal | Food technology and biotechnology
(Food Technol Biotechnol)
Vol. 61
Issue 2
Pg. 191-201
(Jun 2023)
ISSN: 1330-9862 [Print] Croatia |
PMID | 37457905
(Publication Type: Journal Article)
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