Abstract | PURPOSE: METHODS: RESULTS: After showcasing the self-labeling potential of the SNAP-tag component, surface binding and internalization of the fluorescently labeled product were demonstrated on CSPG4-positive TNBC cell lines through confocal microscopy and flow cytometry. The cell-killing ability of the novel AURIF-based recombinant ADC was illustrated by the induction of a 50% reduction in cell viability at nanomolar to micromolar concentrations on target cell lines. CONCLUSION: This research underscores the applicability of SNAP-tag in the unambiguous generation of homogeneous and pharmaceutically relevant immunoconjugates that could potentially be instrumental in the management of a daunting disease like TNBC.
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Authors | Neelakshi Mungra, Fleury A N Biteghe, Zaria Malindi, Allan M Huysamen, Maryam Karaan, Natasha S Hardcastle, Rubina Bunjun, Shivan Chetty, Krupa Naran, Dirk Lang, Wolfgang Richter, Roger Hunter, Stefan Barth |
Journal | Journal of cancer research and clinical oncology
(J Cancer Res Clin Oncol)
Vol. 149
Issue 13
Pg. 12203-12225
(Oct 2023)
ISSN: 1432-1335 [Electronic] Germany |
PMID | 37432459
(Publication Type: Journal Article)
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Copyright | © 2023. The Author(s). |
Chemical References |
- Immunoconjugates
- chondroitin sulfate proteoglycan 4
- auristatin
- Single-Chain Antibodies
- CSPG4 protein, human
- Membrane Proteins
- Chondroitin Sulfate Proteoglycans
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Topics |
- Humans
- Immunoconjugates
(pharmacology, chemistry)
- Triple Negative Breast Neoplasms
(pathology)
- Single-Chain Antibodies
(pharmacology)
- Cell Line, Tumor
- Membrane Proteins
- Chondroitin Sulfate Proteoglycans
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