Abstract | BACKGROUND: METHODS:
CircRNA expression profiles of HCC tissues were jointly analyzed to identify differentially expressed circRNAs. Overexpression plasmid and siRNA targeting candidate circRNAs were used in functional assays in vitro. CircRNA- miRNA interactions were predicted using miRNAs expressed in the miRNA-seq dataset GSE76903. To further screen downstream genes targeted by the miRNAs, survival analysis and qRT-PCR were conducted to evaluate their prognostic role in HCC and construct a ceRNA regulatory network. RESULTS: Three significantly upregulated circRNAs, hsa_circ_0002003, hsa_circ_0002454, and hsa_circ_0001394, and one significantly downregulated circRNA, hsa_circ_0003239, were identified and validated by qRT-PCR. Our in vitro data indicated that upregulation of hsa_circ_0002003 accelerated cell growth and metastasis. Mechanistically, DTYMK, DAP3, and STMN1, which were targeted by hsa-miR-1343-3p, were significantly downregulated in HCC cells when hsa_circ_0002003 was silenced and were significantly correlated with poor prognosis in patients with HCC. CONCLUSION: Hsa_circ_0002003 may play critical roles in HCC pathogenesis and serve as a potential prognostic biomarker for HCC. Targeting the hsa_circ_0002003/hsa-miR-1343-3p/STMN1 regulatory axis could be an effective therapeutic strategy in patients with HCC.
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Authors | Lisha Zhou, Qianwen Wang, Jun Hou, Xiangwei Wu, Lianghai Wang, Xueling Chen |
Journal | BMC cancer
(BMC Cancer)
Vol. 23
Issue 1
Pg. 611
(Jul 03 2023)
ISSN: 1471-2407 [Electronic] England |
PMID | 37400785
(Publication Type: Journal Article)
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Copyright | © 2023. The Author(s). |
Chemical References |
- RNA, Circular
- MicroRNAs
- Biomarkers
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Topics |
- Humans
- Carcinoma, Hepatocellular
(pathology)
- RNA, Circular
(genetics)
- Up-Regulation
- Liver Neoplasms
(pathology)
- MicroRNAs
(metabolism)
- Biomarkers
(analysis)
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