Abstract | BACKGROUND AND PURPOSE: EXPERIMENTAL APPROACH: Male CD-1 mice were injected with vehicle, JWH-018 (6 mg·kg-1 ), the CB1 -antagonist NESS-0327 (1 mg·kg-1 ) or co-administration of NESS-0327 and JWH-018, every day for 7 days. After 15 or 16 days washout, we investigated the effects of JWH-018 on motor function, memory, social dominance and prepulse inhibition (PPI). We also evaluated glutamate levels in dialysates from dorsal striatum, striatal dopamine content and striatal/hippocampal neuroplasticity focusing on the NMDA receptor complex and the neurotrophin BDNF. These measurements were accompanied by in vitro electrophysiological evaluations in hippocampal preparations. Finally, we investigated the density of CB1 receptors and levels of the endocannabinoid anandamide (AEA) and 2-arachidonoylglycerol (2-AG) and their main synthetic and degrading enzymes in the striatum and hippocampus. KEY RESULTS: The repeated treatment with JWH-018 induced psychomotor agitation while reducing social dominance, recognition memory and PPI in mice. JWH-018 disrupted hippocampal LTP and decreased BDNF expression, reduced the synaptic levels of NMDA receptor subunits and decreased the expression of PSD95. Repeated exposure to JWH-018, reduced hippocampal CB1 receptor density and induced a long-term alteration in AEA and 2-AG levels and their degrading enzymes, FAAH and MAGL, in the striatum. CONCLUSION AND IMPLICATIONS: Our findings suggest that repeated administration of a high dose of JWH-018 leads to the manifestation of psychotic-like symptoms accompanied by alterations in neuroplasticity and change in the endocannabinoid system.
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Authors | Sabrine Bilel, Erica Zamberletti, Lucia Caffino, Micaela Tirri, Francesca Mottarlini, Raffaella Arfè, Mario Barbieri, Sarah Beggiato, Federica Boccuto, Tatiana Bernardi, Sara Casati, Anna T Brini, Daniela Parolaro, Tiziana Rubino, Luca Ferraro, Fabio Fumagalli, Matteo Marti |
Journal | British journal of pharmacology
(Br J Pharmacol)
Vol. 180
Issue 21
Pg. 2777-2801
(11 2023)
ISSN: 1476-5381 [Electronic] England |
PMID | 37311647
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2023 The Authors. British Journal of Pharmacology published by John Wiley & Sons Ltd on behalf of British Pharmacological Society. |
Chemical References |
- Endocannabinoids
- 1-pentyl-3-(1-naphthoyl)indole
- Brain-Derived Neurotrophic Factor
- Receptors, N-Methyl-D-Aspartate
- Cannabinoids
- Receptor, Cannabinoid, CB1
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Topics |
- Mice
- Male
- Animals
- Endocannabinoids
(metabolism)
- Brain-Derived Neurotrophic Factor
(metabolism)
- Receptors, N-Methyl-D-Aspartate
- Cannabinoids
(pharmacology)
- Neuronal Plasticity
- Cognitive Dysfunction
- Receptor, Cannabinoid, CB1
(metabolism)
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