HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Linc-ROR Regulates POU5F1 and SOX2 by Competitively Binding miR-145-5p to Affect the Proliferation and Migration of Gastric Cancer Cells.

Abstract
Gastric cancer is the third leading cause of cancer-related deaths worldwide, and research on gastric cancer pathogenesis is fundamental. Long intergenic non-coding RNAs (lincRNAs) control cancer initiation and progression through several mechanisms, with the competitive endogenous RNA (ceRNA) regulatory network being the most common. In this study, in situ hybridization revealed that long intergenic non-protein coding RNA-regulator of reprogramming (linc-ROR) was highly expressed in gastric cancer cells and was mainly cytoplasmic-positive. Cell counting kit-8 (CCK-8), plate colony formation, wound healing, and Transwell assay revealed that linc-ROR knockdown impedes the growth, proliferation, and migration of gastric cancer cells, while linc-ROR overexpression promoted gastric cancer cell growth, migration, and colony formation ability. Combined with previous studies, the molecular mechanism axis of linc-ROR/miR-145-5-5p/POU5F1/SOX2 was verified. The expression of linc-ROR knockdown significantly suppressed the protein expression of POU5F1 and SOX2. Co-transfection with linc-ROR siRNA reverses the carcinogenic effect of the miR-145-5p inhibitor on gastric cancer cell proliferation, cloning, and migration. These findings lay a foundation for developing novel targets for gastric cancer treatment.
AuthorsXiuchong Yu, Xinyi Ma, Yangtao Jia, Xinxin Wu, Zhilong Yan
JournalCell transplantation (Cell Transplant) 2023 Jan-Dec Vol. 32 Pg. 9636897231178902 ISSN: 1555-3892 [Electronic] United States
PMID37306240 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • MicroRNAs
  • POU5F1 protein, human
  • Octamer Transcription Factor-3
  • SOX2 protein, human
  • SOXB1 Transcription Factors
  • MIRN145 microRNA, human
Topics
  • Humans
  • Stomach Neoplasms (genetics)
  • Cytoplasm
  • Cell Count
  • Cell Proliferation (genetics)
  • MicroRNAs (genetics)
  • Octamer Transcription Factor-3
  • SOXB1 Transcription Factors (genetics)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: