Microfluidic Protocols for the Assessment of Anticancer Therapies in 3D Tumor-Stromal Cocultures.

Abstract

Microfluidic technologies allow the generation of large datasets using smaller quantities of cells and reagents than with traditional well plate assays. Such miniaturized methods can also facilitate the generation of complex 3D preclinical models of solid tumors with controlled size and cell composition. This is particularly useful in the context of recreating the tumor microenvironment for preclinical screening of immunotherapies and combination therapies at a scale, to reduce the experimental costs during therapy development while using physiologically relevant 3D tumor models, and to assess the therapy's efficacy. Here, we describe the fabrication of microfluidic devices and the associated protocols to culture tumor-stromal spheroids for assessing the efficacy of anticancer immunotherapies as monotherapies and as part of combination therapy regimes.

• Authors: Karla Paterson, Michele Zagnoni
• Journal: Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol) Vol. 2679 Pg. 127-139 ( 2023) ISSN: 1940-6029 [Electronic] United States
• PMID: 37300612 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Topics

• Coculture Techniques
• Microfluidics (methods)
• Cell Line, Tumor
• Spheroids, Cellular
• Tumor Microenvironment