Impact of 1,6-hexanediol on Schizosaccharomyces pombe genome stability.

Abstract

Phase separation is a major mechanism of macromolecular condensation within cells. A frequently chosen tool for global disruption of phase separation via weak hydrophobic interactions is treatment with 1,6-hexanediol. This study evaluates the cytotoxic and genotoxic effects of treating live fission yeast with 1,6-hexanediol. We find that 1,6-hexanediol causes a drastic decrease in cell survival and growth rate. We also see a reduction in HP1 protein foci and increase in DNA damage foci. However, there is no evidence for increased genomic instability in two classically phase-separated domains, the heterochromatic pericentromere and the nucleolar rDNA repeats. This study reveals that 1,6-hexanediol is a blunt tool for phase separation inhibition and its secondary effects must be taken into consideration during its in vivo use.

• Authors: Chance E Jones, Susan L Forsburg
• Journal: G3 (Bethesda, Md.) (G3 (Bethesda)) Vol. 13 Issue 8 (08 09 2023) ISSN: 2160-1836 [Electronic] England
• PMID: 37284815 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: © The Author(s) 2023. Published by Oxford University Press on behalf of The Genetics Society of America.

Chemical References

• Schizosaccharomyces pombe Proteins
• hexamethylene glycol
• Heterochromatin

Topics

• Humans
• Schizosaccharomyces (metabolism)
• Schizosaccharomyces pombe Proteins (genetics, metabolism)
• Heterochromatin (metabolism)
• Genomic Instability