Exosome GLUT1 derived from hepatocyte identifies the risk of non-alcoholic steatohepatitis and fibrosis.

Abstract

BACKGROUND AND AIMS:

It is particularly important to identify the progression of non-alcoholic fatty liver disease (NAFLD) for prognosis evaluation and treatment guidance. The aim of this study was to explore the clinic use of exosomal protein-based detection as a valuable non-invasive diagnostic method for NAFLD.

METHODS:

Exosomes were extracted from plasma of patients with NAFLD using Optima XPN-100 ultrafast centrifuge. The patients were recruited from outpatients and inpatients of Beijing Youan Hospital Affiliated to Capital Medical University. The exosomes were stained with fluorescent-labeled antibody and determined by ImageStream® X MKII imaging flow cytometry. Generalized linear logistic regression model was used to evaluate the diagnostic value of hepatogenic exosomes in NAFLD and liver fibrosis.

RESULTS:

The percentage of hepatogenic exosomes glucose transporter 1 (GLUT1) in patients with non-alcoholic steatohepatitis (NASH) was significantly higher than that in patients with non-alcoholic fatty liver (NAFL). According to liver biopsy, we found that the percentage of hepatogenic exosomes GLUT1 in patients with advanced NASH (F2-4) was significantly higher than that in patients with early NASH (F0-1), and the same trend was observed in exosomes with CD63 and ALB. Compared with other clinical fibrosis scoring criteria (FIB-4, NFS, etc.), the diagnostic performance of hepatogenic exosomes GLUT1 was the highest and the area under the receiver-operating curves (AUROC) was 0.85 (95% CI 0.77-0.93). Furthermore, the AUROC of hepatogenic exosomes GLUT1 combined with fibrosis scoring was as high as 0.86-0.91.

CONCLUSION:

Hepatogenic exosome GLUT1 can be a molecular biomarker for early warning of NAFLD to distinguish the NAFL and NASH, and it also can be used as a novel non-invasive diagnostic biomarker for the staging liver fibrosis in NAFLD.

• Authors: Wenyan Zhang, Jing Zhang, Honglin Shi, Fang Liu, Haibin Yu, Hongbo Shi
• Journal: Hepatology international (Hepatol Int) Vol. 17 Issue 5 Pg. 1170-1181 (Oct 2023) ISSN: 1936-0541 [Electronic] United States
• PMID: 37278869 (Publication Type: Journal Article)
• Copyright: © 2023. Asian Pacific Association for the Study of the Liver.

Chemical References

• Glucose Transporter Type 1
• Biomarkers

Topics

• Humans
• Non-alcoholic Fatty Liver Disease (pathology)
• Glucose Transporter Type 1
• Exosomes (pathology)
• Fibrosis
• Liver Cirrhosis (pathology)
• Biomarkers
• Hepatocytes (pathology)
• Biopsy (methods)
• Liver (pathology)