Abstract | Background: Hepatic lipid disorder impaired mitochondrial homeostasis and intracellular redox balance, triggering development of non- alcohol fatty liver disease ( NAFLD), while effective therapeutic approach remains inadequate. Ginsenosides Rc has been reported to maintain glucose balance in adipose tissue, while its role in regulating lipid metabolism remain vacant. Thus, we investigated the function and mechanism of ginsenosides Rc in defending high fat diet (HFD)-induced NAFLD. Methods: Mice primary hepatocytes (MPHs) challenged with oleic acid & palmitic acid were used to test the effects of ginsenosides Rc on intracellular lipid metabolism. RNAseq and molecular docking study were performed to explore potential targets of ginsenosides Rc in defending lipid deposition. Wild type and liver specific sirtuin 6 (SIRT6, 50721) deficient mice on HFD for 12 weeks were subjected to different dose of ginsenosides Rc to determine the function and detailed mechanism in vivo. Results: Conclusion:
Ginsenosides Rc protects mice against HFD-induced hepatosteatosis by improving PPAR-α-mediated fatty acid oxidation and antioxidant capacity in a SIRT6 dependent manner, and providing a promising strategy for NAFLD.
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Authors | Zehong Yang, Yuanyuan Yu, Nannan Sun, Limian Zhou, Dong Zhang, HaiXin Chen, Wei Miao, Weihang Gao, Canyang Zhang, Changhui Liu, Xiaoying Yang, Xiaojie Wu, Yong Gao |
Journal | Journal of ginseng research
(J Ginseng Res)
Vol. 47
Issue 3
Pg. 376-384
(May 2023)
ISSN: 1226-8453 [Print] Korea (South) |
PMID | 37252281
(Publication Type: Journal Article)
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Copyright | © 2023 The Korean Society of Ginseng. Publishing services by Elsevier B.V. |