Paeonol Promotes Reendothelialization After Vascular Injury Through Activation of c-Myc/VEGFR2 Signaling Pathway.

Abstract

Purpose:

Dysfunction of endothelium is associated with multiple pathological vascular diseases. However, how to regulate reendothelialization after vascular injury is not well defined. This study aims to determine whether and how Paeonol controls reendothelialization following artery injury.

Methods:

The endothelium of murine carotid artery was denuded by catheter guide wires injury. H&E staining and IF staining were performed to determine whether Paeonol is critical for reendothelialization. BRDU Incorporation Assay, Boyden Chamber Migration Assay, Tube Formation Assay, and Spheroid Sprouting Assay were used to investigate whether Paeonol is involved in regulating proliferation and migration of endothelial cells. The underlying mechanism of how Paeonol regulates reendothelialization was determined by Molecular docking simulation and CO-IP Assay.

Results:

Paeonol treatment significantly inhibits neointima formation in carotid artery ligation model by promoting proliferation and migration of endothelial cells. Mechanistically, Paeonol enhances c-Myc expression, consequently interacts with VEGFR2 results in activating VEGF signaling pathway, and eventually promotes reendothelialization after vascular injury.

Conclusion:

Our data demonstrated that Paeonol plays a critical role in regulating vascular reendothelialization, which may be therapeutically used for treatment of pathological vascular diseases.

• Authors: Yang Wang, Zheng Wang, Xiao Wu, Song Zhu, Qiru Guo, Zhong Jin, Zixian Chen, Delai Zhang, Wangming Hu, Huan Xu, Liangqin Shi, Lan Yang, Yong Wang
• Journal: Drug design, development and therapy (Drug Des Devel Ther) Vol. 17 Pg. 1567-1582 ( 2023) ISSN: 1177-8881 [Electronic] New Zealand
• PMID: 37249931 (Publication Type: Journal Article)
• Copyright: © 2023 Wang et al.

Chemical References

• paeonol

Topics

• Mice
• Animals
• Vascular System Injuries (drug therapy, metabolism)
• Endothelial Cells (metabolism)
• Endothelium, Vascular (metabolism)
• Carotid Artery Injuries (drug therapy, pathology)
• Molecular Docking Simulation
• Signal Transduction
• Cells, Cultured