The previous research has focused on the suppressive effects of
Erianin on
tumor progression, but its impact on
cancer stemness has not been reported. This study aimed to investigate the effects of
Erianin on
lung cancer stemness. First, we screened various concentrations
Erianin to ensure that it did not affect
lung cancer cell viability. Subsequently, we found that
Erianin significantly attenuated
lung cancer stemness through various analyses, including qRT-PCR, western blot, sphere-formation, and ALDH activity detection. Furthermore,
Erianin was shown to enhance chemosensitivity of
lung cancer cells. Mechanistically, three inhibitors (cell apoptosis inhibitor,
necrosis inhibitor, and ferroptosis inhibitor) were added into
lung cancer cells with
Erianin treatment, respectively, and we found that
Erianin mainly suppressed
lung cancer stemness through ferroptosis. Taken together, this study reveals that
Erianin has the potential to suppress
lung cancer stemness and could be a valuable chemotherapeutic enhancer for
lung cancer.