Design and synthesis of salidroside analogs and their bioactivity against septic myocardial injury.

Abstract

Cardiac tissue suffers much from sepsis, and the incidence of myocardial injury is high in septic patients. The treatment of sepsis myocardial injury (SMI) has been the focus of clinical medicine. Salidroside shows myocardial cell protection, anti-oxidation and anti- inflammation effects, and it is thought as one of the potential compounds to treat sepsis myocardial injury. However, its anti-inflammatory activity is lower and its pharmacokinetic properties are not ideal, which is far from clinical application. Here, a series of salidroside analogs were synthesized, and their bioactivities were evaluated from several aspects, including their anti-oxidant and anti-inflammatory activities in vitro and anti-sepsis myocardial injury activities in vivo. Of all the compounds which synthesized, compounds 2 and 3 exhibited stronger anti-inflammatory activities than the others; after treating LPS-stimulated RAW264.7 or H9c2 cells with each of them, the levels of IL-1β, IL-6 and TNF-α were down-regulated in a dose-dependent manner. In the anti-oxidative stress injury test, compounds 2 and 3 not only markedly increased the survival rate of cells, and but also improved the cellular oxidative stress-related indicators MDA, SOD and cell damage marker LDH in a dose-dependent manner. In the LPS-induced septic rat myocardial injury models (in vivo), the two compounds also showed good bioactivities. They also reduced the expression of IL-1β, IL-6 and TNF-α, and blocked cell damage by suppressing overhauled oxidation in septic rats. In addition, the myocardial injury was significantly improved and the inflammatory infiltration was reduced after treatment with the two compounds. In conclusion, the salidroside analogs (2 and 3) showed promising therapeutical effect on septic myocardial injury in LPS-model rats, and they could be good candidates for clinical trials against inflammation and septic myocardial injury.

• Authors: Zongyuan Wang, Xin Qiang, Yijie Peng, Wenjie Fu, Quanyi Zhao, Dian He
• Journal: Bioorganic chemistry (Bioorg Chem) Vol. 138 Pg. 106609 (09 2023) ISSN: 1090-2120 [Electronic] United States
• PMID: 37207595 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2023 Elsevier Inc. All rights reserved.

Chemical References

• rhodioloside
• Tumor Necrosis Factor-alpha
• Interleukin-6
• Lipopolysaccharides
• Anti-Inflammatory Agents

Topics

• Rats
• Animals
• Tumor Necrosis Factor-alpha (metabolism)
• Interleukin-6 (metabolism)
• Lipopolysaccharides (pharmacology)
• Anti-Inflammatory Agents (pharmacology)
• Sepsis (drug therapy)
• Inflammation