Cowanin induces apoptosis in breast cancer cells via Bcl-2 signaling pathway.

Abstract

OBJECTIVES:

This study aimed to investigate the effect of cowanin the mechanism of cowanin toward cell death and BCL-2 protein (antiapoptotic) expression of T47D breast cancer.

METHODS:

The cell death was evaluated by double staining, namely acridine orange and propidium iodide, and then observed under a fluorescence microscope. Meanwhile, the BCL-2 protein expression was determined by western blotting with measurement of protein area and protein density.

RESULTS:

The result found T47D breast cancer cells were viable, apoptosis, and necrosis after treatment with cowanin. The average viable cells, apoptosis, and necrosis percentages were 54.13 %, 45.43 %, and 0.44 %, respectively. Statistical analysis showed cowanin could significantly induce death in T47D breast cancer cells by apoptosis (p<0.05). It was also revealed that cowanin and positive control (doxorubicin) treatment had a significantly decreased protein area and protein density (p<0.05).

CONCLUSIONS:

It can be concluded that cowanin can induce death in T47D breast cancer cells by apoptosis and affect the expression of Bcl-2 protein in T47D breast cancer cells.

• Authors: Fatma Sri Wahyuni, Suryati Syafri, Dita Permatasari, Dira Hefni, Dachriyanus Hamidi, Nur Azizah Nomiza, Indri Aulia Rezti
• Journal: Journal of complementary & integrative medicine (J Complement Integr Med) Vol. 20 Issue 3 Pg. 631-636 (Sep 01 2023) ISSN: 1553-3840 [Electronic] Germany
• PMID: 37202914 (Publication Type: Journal Article)
• Copyright: © 2023 Walter de Gruyter GmbH, Berlin/Boston.

Chemical References

• cowanin
• Proto-Oncogene Proteins c-bcl-2

Topics

• Humans
• Apoptosis
• Necrosis
• Signal Transduction
• Proto-Oncogene Proteins c-bcl-2
• Neoplasms