Gleason Score-related MT1L as biomarker for prognosis in prostate adenocarcinoma and contribute to tumor progression in vitro.

Abstract

BACKGROUND:

The Gleason Score is well correlated with biological behavior and prognosis in prostate adenocarcinoma (PRAD). This study was derived to determine the clinical significance and function of Gleason-Score-related genes in PRAD.

METHODS:

RNA-sequencing profiles and clinical data were extracted from the The Cancer Genome Atlas PRAD database. The Gleason-Score-related genes were screened out by the Jonckheere-Terpstra rank-based test. The "limma" R package was performed for differentially expressed genes. Next, a Kaplan-Meier survival analysis was performed. Correlation MT1L expression levels with tumor stage, non-tumor tissue stage, radiation therapy, and residual tumor were analyzed. Further, MT1L expression was detected in PRAD cell lines by reverse transcription-quantitative polymerase chain reaction assay. Overexpression of MT1L was constructed and used for cell count kit-8, flow cytometric assay, transwell assay, and wound-healing assay.

RESULTS:

Survival analysis showed 15 Gleason-Score-related genes as prognostic biomarkers in PRAD. The high-frequency deletion of MT1L was verified in PRAD. Furthermore, MT1L expression was decreased in PRAD cell lines than RWPE-1 cells, and overexpression of MT1L repressed cell proliferation and migration, and induced apoptosis in PC-3 cells.

CONCLUSION:

Gleason-Score-related MT1L may serve as a biomarker of poor prognostic biomarker in PRAD. In addition, MT1L plays a tumor suppressor in PRAD progression, which is beneficial for PRAD diagnosis and treatment research.

• Authors: Lei Liu, Yaping Li, Shiying Tang, Bin Yang, Qiming Zhang, Ruotao Xiao, Xiaofei Hou, Cheng Liu, Lulin Ma
• Journal: The International journal of biological markers (Int J Biol Markers) Vol. 38 Issue 2 Pg. 114-123 (Jun 2023) ISSN: 1724-6008 [Electronic] United States
• PMID: 37192745 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor

Topics

• Male
• Humans
• Neoplasm Grading
• Prostate (metabolism, pathology)
• Prognosis
• Prostatic Neoplasms (genetics, pathology)
• Biomarkers, Tumor (genetics, metabolism)
• Adenocarcinoma (pathology)