[Capacity of the polynucleotide phosphorylase-releasing factor to stimulate hybrid cell growth].

Abstract

The capacity of the partially purified preparation of the polynucleotide phosphorylase releasing factor (PNP-ase RF) from Walker sarcoma for stimulating the growth of hybrid cells and parent myeloma cells has been studied. The preparation of PNP-ase RF at a concentration of 500-50 micrograms/ml has been shown to induce the 3- to 10-fold increase of the number of cells in the culture by the 5th day of incubation without changing the medium.

• Authors: A P Tarasov, V V Sviridov
• Journal: Zhurnal mikrobiologii, epidemiologii i immunobiologii (Zh Mikrobiol Epidemiol Immunobiol) Issue 4 Pg. 88-9 (Apr 1986) ISSN: 0372-9311 [Print] Russia (Federation)
• Vernacular Title: Izuchenie sposobnosti faktora sniatiia polinukleotidfosforilazy stimulirovat' rost kletochnykh gibridov.
• PMID: 3716692 (Publication Type: Comparative Study, English Abstract, Journal Article)

Chemical References

• Glycoproteins
• Neoplasm Proteins
• Tissue Extracts
• Polyribonucleotide Nucleotidyltransferase

Topics

• Animals
• Dose-Response Relationship, Drug
• Glycoproteins (pharmacology)
• Hybrid Cells (drug effects)
• Hybridomas (drug effects)
• Liver
• Methods
• Molecular Weight
• Multiple Myeloma (physiopathology)
• Neoplasm Proteins (isolation & purification, pharmacology)
• Polyribonucleotide Nucleotidyltransferase (metabolism)
• Rats
• Sarcoma, Experimental (metabolism)
• Stimulation, Chemical
• Time Factors
• Tissue Extracts (pharmacology)