Impacts of potential anticancer agents based on pillar[5]arene for head and neck squamous cell carcinoma cells.

Abstract

PURPOSE:

This study was conducted to investigate impacts of potential anticancer (associated with apoptosis and caspase pathways) of two newly synthesized derivatives of pillar[5]arene, named as d-Q-P5 and p-Q-P5, on Squamous cell carcinomas of the head and neck (HNSCC) cells.

MATERIALS AND METHODS:

The MTT method was used to determine the IC50 doses of the derivatives on HNSCC cells, and the changes in gene expression were analyzed by real-time polymerase chain reaction (qPCR). The apoptosis change was confirmed by flow cytometry analysis.

RESULTS:

The results showed that the d-Q-P5 and p-Q-P5 effectively inhibited the proliferation of the cells by upregulating proapoptotic genes (Bax, Bad, p53, Bak, and Apaf-1) and genes involved in the caspase pathway (Casp2, Casp3, and Casp9), while downregulating the antiapoptotic gene (Bcl-2).

CONCLUSIONS:

This study is the first to demonstrate the potential anticancer effects of these two agents on HNSCC cells by positively regulating apoptosis gene expression.

• Authors: Serkan Kuccukturk, Mehmet Ali Karaselek, Tugce Duran, Ahmed Nuri Kursunlu, Mustafa Ozmen
• Journal: Journal of cancer research and clinical oncology (J Cancer Res Clin Oncol) Vol. 149 Issue 11 Pg. 8711-8718 (Sep 2023) ISSN: 1432-1335 [Electronic] Germany
• PMID: 37126106 (Publication Type: Journal Article)
• Copyright: © 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Chemical References

• pillar(5)arene
• Caspases
• Antineoplastic Agents

Topics

• Humans
• Squamous Cell Carcinoma of Head and Neck (drug therapy, genetics)
• Head and Neck Neoplasms (drug therapy, genetics)
• Apoptosis
• Caspases
• Antineoplastic Agents (pharmacology)
• Cell Line, Tumor