Abstract |
In this study, peripheral blood mononuclear cells from young and old patients with COVID-19 were examined phenotypically, transcriptionally and functionally to reveal age-, time- and severity-specific adaptations. Gene signatures within memory B cells and plasmablasts correlated with reduced frequency of antigen-specific B cells and neutralizing antibodies in older patients with severe COVID-19. Moreover, these patients exhibited exacerbated T cell lymphopenia, which correlated with lower plasma interleukin-2, and diminished antigen-specific T cell responses. Single-cell RNA sequencing revealed augmented signatures of activation, exhaustion, cytotoxicity and type I interferon signaling in memory T and natural killer cells with age. Although cytokine storm was evident in both age groups, older individuals exhibited elevated levels of myeloid cell recruiting factors. Furthermore, we observed redistribution of monocyte and dendritic cell subsets and emergence of a suppressive phenotype with severe disease, which was reversed only in young patients over time. This analysis provides new insights into the impact of aging on COVID-19.
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Authors | Sloan A Lewis, Suhas Sureshchandra, Michael Z Zulu, Brianna Doratt, Allen Jankeel, Izabela Coimbra Ibraim, Amanda N Pinski, Nicholas S Rhoades, Micaila Curtis, Xiwen Jiang, Delia Tifrea, Frank Zaldivar, Weining Shen, Robert A Edwards, Daniel Chow, Dan Cooper, Alpesh Amin, Ilhem Messaoudi |
Journal | Nature aging
(Nat Aging)
Vol. 1
Issue 11
Pg. 1038-1052
(11 2021)
ISSN: 2662-8465 [Electronic] United States |
PMID | 37118336
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Copyright | © 2021. The Author(s), under exclusive licence to Springer Nature America, Inc. |
Topics |
- Humans
- Leukocytes, Mononuclear
- COVID-19
- SARS-CoV-2
- Acclimatization
- Immunity
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