Preeclampsia (PE) is a common
pregnancy complication, and placental
hypoxia is one of its causes. We aimed to identify the transcriptional profile and construct a long non-coding RNAs (
lncRNA)-centered competing endogenous RNAs (
ceRNA) network in
hypoxia-induced HTR8/SVneo cells. We used datasets from the GEO database to identify important pathways in PE. We performed microarray profiling and functional analysis to identify differentially expressed long non-coding RNAs (lncRNAs), differentially expressed profiles of
microRNA (
miRNAs), and differentially expressed profiles of
messenger RNA (mRNAs) in
hypoxia-induced HTR8/SVneo cells. The candidates were validated using quantitative reverse transcription polymerase chain reaction. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes analyses were performed to understand the functional significance of differentially expressed genes. Finally, we constructed an
lncRNA-centered
ceRNA network. Several hub genes were validated both in placentas from PE and normal pregnancy, and in
hypoxia-induced HTR8/SVneo cells. The hypoxic response pathway was involved in the pathophysiology of PE. Subsequently, we identified 536 differentially expressed profiles of lncRNAs (183 upregulated and 353 downregulated), 46 differentially expressed profiles of
miRNAs (35 upregulated and 11 downregulated), and 2782 differentially expressed profiles of mRNAs (DEmRNAs) (1031 upregulated and 1751 downregulated) in
hypoxia-induced HTR8/SVneo cells. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed potential pathways affected by these genes, such as angiogenesis, the HIF-1 signaling pathway, and the PI3K-Akt signaling pathway. The
ceRNA network comprised 35 lncRNAs, 11
miRNAs, 27 mRNAs, and 2 hub lncRNAs, which might play a vital role in placental functions and PE. Our results revealed the transcriptome profile and constructed an
lncRNA-centered
ceRNA network in
hypoxia-induced HTR8/SVneo cells, thereby providing potential therapeutic targets for PE.