Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives.

Abstract

The post-synaptic density protein 95 (PSD95) is a crucial scaffolding protein participating in the organization and regulation of synapses. PSD95 interacts with numerous molecules, including neurotransmitter receptors and ion channels. The functional dysregulation of PSD95 as well as its abundance and localization has been implicated with several neurological disorders, making it an attractive target for developing strategies able to monitor PSD95 accurately for diagnostics and therapeutics. This study characterizes a novel camelid single-domain antibody (nanobody) that binds strongly and with high specificity to rat, mouse, and human PSD95. This nanobody allows for more precise detection and quantification of PSD95 in various biological samples. We expect that the flexibility and unique performance of this thoroughly characterized affinity tool will help to further understand the role of PSD95 in normal and diseased neuronal synapses.

• Authors: Markus Kilisch, Maja Gere-Becker, Liane Wüstefeld, Christel Bonnas, Alexander Crauel, Maja Mechmershausen, Henrik Martens, Hansjörg Götzke, Felipe Opazo, Steffen Frey
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 24 Issue 8 (Apr 14 2023) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 37108454 (Publication Type: Journal Article)

Chemical References

• Disks Large Homolog 4 Protein
• Ion Channels
• Transcription Factors

Topics

• Rats
• Mice
• Humans
• Animals
• Disks Large Homolog 4 Protein (metabolism)
• Synapses (metabolism)
• Neurons (metabolism)
• Post-Synaptic Density (metabolism)
• Ion Channels (metabolism)
• Transcription Factors (metabolism)