Evaluation of catalytic activity of human and animal origin viral neuraminidase: Current prospect.

Abstract

With the exception of plants, almost all living organisms synthesize neuraminidase/sialidase. It is a one among the crucial proteins that controls how virulent a microorganism is. An essential enzyme in orthomyxoviruses and paramyxoviruses that destroys receptors is neuraminidase. It plays a number of roles throughout the viral life cycle in addition to one that involves the release of progeny virus particles. This protein is an important target for therapeutic interventions and diagnostic assays. Neuraminidase inhibitors effectively prevent the spread of disease and viral infection. Sensitive, quick, and inexpensive high throughput assays are needed to screen for specific neuraminidase inhibitory chemicals. To characterize the neuraminidase catalytic activity, however, the traditional assays are still the most common in laboratories. This review gives a brief overview of these neuraminidase assays and recent, innovative developments, particularly those involving biosensors.

• Authors: Anil Gattani, Aditya Agrawal, M Hira Khan, Rohini Gupta, Praveen Singh
• Journal: Analytical biochemistry (Anal Biochem) Vol. 671 Pg. 115157 (06 15 2023) ISSN: 1096-0309 [Electronic] United States
• PMID: 37061113 (Publication Type: Journal Article, Review)
• Copyright: Copyright © 2023 Elsevier Inc. All rights reserved.

Chemical References

• Neuraminidase
• Antiviral Agents
• Enzyme Inhibitors
• Guanidines

Topics

• Animals
• Humans
• Neuraminidase
• Antiviral Agents
• Enzyme Inhibitors (pharmacology, chemistry)
• Orthomyxoviridae
• Guanidines