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Inhibitory Effects of 3-Cyclopropylmethoxy-4-(difluoromethoxy) Benzoic Acid on TGF-β1-Induced Epithelial-Mesenchymal Transformation of In Vitro and Bleomycin-Induced Pulmonary Fibrosis In Vivo.

Abstract
Idiopathic pulmonary fibrosis (IPF) is a progressive lung disease characterized by lung inflammation and excessive deposition of extracellular matrix components. Transforming growth factor-β1 (TGF-β1) induced epithelial-mesenchymal transformation of type 2 lung epithelial cells leads to excessive extracellular matrix deposition, which plays an important role in fibrosis. Our objective was to evaluate the effects of 3-cyclopropylmethoxy-4-(difluoromethoxy) benzoic acid (DGM) on pulmonary fibrosis and aimed to determine whether EMT plays a key role in the pathogenesis of pulmonary fibrosis and whether EMT can be used as a therapeutic target for DGM therapy to reduce IPF. Firstly, stimulation of in vitro cultured A549 cells to construct EMTs with TGF-β1. DGM treatment inhibited the expression of proteins such as α-SMA, vimentin, and collagen Ⅰ and increased the expression of E-cadherin. Accordingly, Smad2/3 phosphorylation levels were significantly reduced by DGM treatment. Secondly, models of tracheal instillation of bleomycin and DGM were used to treat rats to demonstrate their therapeutic effects, such as improving lung function, reducing lung inflammation and fibrosis, reducing collagen deposition, and reducing the expression of E-cadherin. In conclusion, DGM attenuates TGF-β1-induced EMT in A549 cells and bleomycin-induced pulmonary fibrosis in rats.
AuthorsTianxiao Sun, Haihua Li, Yan Zhang, Guixin Xiong, Yuerun Liang, Fang Lu, Rong Zheng, Qi Zou, Jiejie Hao
JournalInternational journal of molecular sciences (Int J Mol Sci) Vol. 24 Issue 7 (Mar 24 2023) ISSN: 1422-0067 [Electronic] Switzerland
PMID37047142 (Publication Type: Journal Article)
Chemical References
  • Transforming Growth Factor beta1
  • Bleomycin
  • Benzoic Acid
  • Collagen
  • Cadherins
Topics
  • Rats
  • Animals
  • Transforming Growth Factor beta1 (metabolism)
  • Bleomycin (toxicity)
  • Epithelial-Mesenchymal Transition
  • Benzoic Acid (pharmacology)
  • Fibrosis
  • Idiopathic Pulmonary Fibrosis
  • Collagen (metabolism)
  • Cadherins (metabolism)

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