Polyphyllin B (PPB) is a compound with anti-
tumor effects. Nuclear paraspeckle assembly transcript 1 (NEAT1) is a long-stranded
noncoding RNA that induces epithelial-mesenchymal transition (EMT) of
tumor cells and promotes
tumor growth and
metastasis. However, the role and mechanism of PPB on
melanoma and the correlation between them remain unclear. In this study we screened NEAT1 by using
LncRNA transcriptomic sequencing, and then transfected B16F10 cells using OVER-NEAT1 lentivirus. Next, we found that PPB had significant proliferation inhibition of
melanoma and B16F10 cells through MTT assay and establishment of mouse subcutaneous
transplantation tumor model; in addition, through wound healing assay, transwell assay and establishment of mouse
melanoma lung
metastasis model, we found that PPB significantly inhibited the invasion and migration of B16F10 cells in vitro, and inhibited the
metastasis of
melanoma to lung, bone and liver in vivo. Finally, changes in the expression levels of EMT-related
proteins were assessed by western blot (WB) and immunohistochemistry, and PPB significantly downregulated the expression levels of MMP-9,
N-cadherin, etc., and upregulated
E-cadherin. While overexpressed NEAT1 showed the ability to promote
melanoma proliferation, migration and invasion, in addition to partially reversed the inhibition of proliferation, migration and invasion of
melanoma by PPB mentioned above.