Evaluation and Monitoring of the Natural Toxin Ptaquiloside in Bracken Fern, Meat, and Dairy Products.

Abstract

Ptaquiloside, a naturally occurring cancer-causing substance in bracken fern, has been detected in the meat and milk of cows fed a diet containing bracken fern. A rapid and sensitive method for the quantitative analysis of ptaquiloside in bracken fern, meat, and dairy products was developed using the QuEChERS method and liquid chromatography-tandem mass spectrometry. The method was validated according to the Association of Official Analytical Chemists guidelines and met the criteria. A single matrix-matched calibration method with bracken fern has been proposed, which is a novel strategy that uses one calibration for multiple matrices. The calibration curve ranged from 0.1 to 50 µg/kg and showed good linearity (r2 > 0.99). The limits of detection and quantification were 0.03 and 0.09 µg/kg, respectively. The intraday and interday accuracies were 83.5-98.5%, and the precision was <9.0%. This method was used for the monitoring and exposure assessment of ptaquiloside in all routes of exposure. A total of 0.1 µg/kg of ptaquiloside was detected in free-range beef, and the daily dietary exposure of South Koreans to ptaquiloside was estimated at up to 3.0 × 10-5 µg/kg b.w./day. The significance of this study is to evaluate commercially available products in which ptaquiloside may be present, to monitor consumer safety.

• Authors: Hana Park, Yoeseph Cho, JiEun Lee, Kang Mi Lee, Ho Jun Kim, Jaeick Lee, Yong-Sun Bahn, Junghyun Son
• Journal: Toxins (Toxins (Basel)) Vol. 15 Issue 3 (03 21 2023) ISSN: 2072-6651 [Electronic] Switzerland
• PMID: 36977122 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ptaquiloside
• Sesquiterpenes

Topics

• Animals
• Cattle
• Pteridium
• Sesquiterpenes (analysis)
• Milk (chemistry)
• Meat (analysis)