Abstract | BACKGROUND/AIM: MATERIALS AND METHODS: SOX10 overexpression and knockdown was performed using SOX10 gene transfection and SOX10 siRNA transfection into A375 melanoma cells. PD-L1 expression was assessed by flow cytometry and western blotting. T cell response was evaluated using NY-ESO-1 specific TCR-transduced T (TCR-T) cells by IFNγ ELISPOT assay. RESULTS: SOX10 overexpression increased the expression of PD-L1, whereas SOX10 knockdown, using siRNA, decreased its expression. IFNγ ELISPOT assay revealed that overexpression of SOX10 decreased the susceptibility of cells to NY-ESO-1-specific TCR-T cells. CONCLUSION: SOX10 has a role in the intrinsic immune suppressive mechanisms of melanoma through expression of PD-L1.
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Authors | Kenta Sasaki, Yoshihiko Hirohashi, Kenji Murata, Tomoyuki Minowa, Munehide Nakatsugawa, Aiko Murai, Yuka Mizue, Terufumi Kubo, Takayuki Kanaseki, Tomohide Tsukahara, Sadahiro Iwabuchi, Shinichi Hashimoto, Hisashi Uhara, Akemi Ishida-Yamamoto, Toshihiko Torigoe |
Journal | Anticancer research
(Anticancer Res)
Vol. 43
Issue 4
Pg. 1477-1484
(Apr 2023)
ISSN: 1791-7530 [Electronic] Greece |
PMID | 36974807
(Publication Type: Journal Article)
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Copyright | Copyright © 2023 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. |
Chemical References |
- Immune Checkpoint Proteins
- B7-H1 Antigen
- Ligands
- Programmed Cell Death 1 Receptor
- Receptors, Antigen, T-Cell
- SOX10 protein, human
- SOXE Transcription Factors
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Topics |
- Humans
- Immune Checkpoint Proteins
- T-Lymphocytes
(metabolism)
- B7-H1 Antigen
(genetics)
- Ligands
- Programmed Cell Death 1 Receptor
(metabolism)
- Melanoma
(metabolism)
- Receptors, Antigen, T-Cell
- SOXE Transcription Factors
(genetics)
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