Objective: Study the repair effect of tilapia
collagen on acute
wounds, and the effect on the expression level of related genes and its metabolic direction in the repair process. Materials and methods: After the full-thickness skin defect model was constructed in standard deviation rats, the wound healing effect was observed and evaluated by means of characterization, histology, and immunohistochemistry. RT-PCR, fluorescence tracer, frozen section and other techniques were used to observe the effect of fish
collagen on the expression of related genes and its metabolic direction in the process of
wound repair. Results: After implantation, there was no immune rejection reaction, fish
collagen fused with new
collagen fibers in the early stage of
wound repair, and was gradually degraded and replaced by new
collagen in the later stage. It has excellent performance in inducing vascular growth, promoting
collagen deposition and maturation, and re-epithelialization. The results of fluorescent tracer showed that fish
collagen was decomposed, and the decomposition products were involved in the
wound repair process and remained at the
wound site as a part of the new tissue. RT-PCR results showed that, without affecting
collagen deposition, the expression level of
collagen-related genes was down-regulated due to the implantation of fish
collagen. Conclusion: Fish
collagen has good biocompatibility and
wound repair ability. It is decomposed and utilized in the process of
wound repair to form new tissues.