Bacterial biofilms generally contribute to
chronic infections, including
wound infections. Due to the antibiotic resistance mechanisms protecting bacteria living in the biofilm, they are a serious problem in the wound healing process. To accelerate the wound healing process and avoid
bacterial infection, it is necessary to select the appropriate dressing material. In this study, the promising therapeutic properties of
alginate lyase (AlgL) immobilised on BC membranes for protecting
wounds from
Pseudomonas aeruginosa infection were investigated. The AlgL was immobilised on never dried BC pellicles via physical adsorption. The maximum adsorption capacity of AlgL was 6.0 mg/g of dry BC, and the equilibrium was reached after 2 h. The adsorption kinetics was studied, and it has been proven that the adsorption was consistent with Langmuir isotherm. In addition, the impact of
enzyme immobilisation on bacterial biofilm stability and the effect of simultaneous immobilisation of AlgL and
gentamicin on the viability of bacterial cells was investigated. The obtained results showed that the AlgL immobilisation significantly reduced the amount of
polysaccharides component of the P. aeruginosa biofilm. Moreover, the biofilm disruption by AlgL immobilised on BC membranes exhibited synergism with the
gentamicin, resulting in 86.5% more dead P. aeruginosa PAO-1 cells.