Oxidized
low-density lipoproteins (oxLDLs) induce oxidative stress in the liver tissue, leading to hepatic steatosis,
inflammation, and
fibrosis. Precise information on the role of
oxLDL in this process is needed to establish strategies for the prevention and management of
non-alcoholic fatty liver disease (
NAFLD) and non-
alcoholic steatohepatitis (NASH). Here, we report the effects of native
LDL (nLDL) and
oxLDL on lipid metabolism, lipid droplet formation, and gene expression in a human liver-derived C3A cell line. The results showed that nLDL induced lipid droplets enriched with
cholesteryl ester (CE) and promoted
triglyceride hydrolysis and inhibited oxidative degeneration of CE in association with the altered expression of LIPE, FASN, SCD1, ATGL, and CAT genes. In contrast,
oxLDL showed a striking increase in lipid droplets enriched with CE hydroperoxides (
CE-OOH) in association with the altered expression of SREBP1, FASN, and DGAT1.
Phosphatidylcholine (PC)-OOH/PC was increased in
oxLDL-supplemented cells as compared with other groups, suggesting that oxidative stress increased hepatocellular damage. Thus, intracellular lipid droplets enriched with
CE-OOH appear to play a crucial role in
NAFLD and NASH, triggered by
oxLDL. We propose
oxLDL as a novel therapeutic target and candidate
biomarker for
NAFLD and NASH.