Ginsenoside Re attenuates myocardial ischemia/reperfusion induced ferroptosis via miR-144-3p/SLC7A11.

Abstract

BACKGROUND:

Ginsenoside Re is an active component in ginseng that confers protection against myocardial ischemia/reperfusion (I/R) injury. Ferroptosis is a type of regulated cell death found in various diseases.

PURPOSE:

Our study aims to investigate the role of ferroptosis and the protective mechanism of Ginsenoside Re in myocardial ischemia/reperfusion.

METHODS:

In the present study, we treated rats for five days with Ginsenoside Re, then established the myocardial ischemia/reperfusion injury rat model to detect molecular implications in myocardial ischemia/reperfusion regulation and to determine the underlying mechanism.

RESULTS:

This study identifies the mechanism behind ginsenoside Re's effect on myocardial ischemia/reperfusion injury and its regulation of ferroptosis through miR-144-3p. Ginsenoside Re significantly reduced cardiac damage caused by ferroptosis during myocardial ischemia/reperfusion injury and glutathione decline. To determine how Ginsenoside Re regulated ferroptosis, we isolated exosomes from VEGFR2+ endothelial progenitor cells after ischemia/reperfusion injury and performed miRNA profiling to screen the miRNAs aberrantly expressed in the process of myocardial ischemia/reperfusion injury and ginsenoside Re treatment. We identified that miR-144-3p was upregulated in myocardial ischemia/reperfusion injury by luciferase report and qRT-PCR. We further confirmed that the solute carrier family 7 member 11 (SLC7A11) was the target gene of miR-144-3p by database analysis and western blot. In comparison with ferropstatin-1, a ferroptosis inhibitor, in vivo studies confirmed that ferropstatin-1 also diminished myocardial ischemia/reperfusion injury induced cardiac function damage.

CONCLUSION:

We demonstrated that ginsenoside Re attenuates myocardial ischemia/reperfusion induced ferroptosis via miR-144-3p/SLC7A11.

• Authors: Jian Ye, Tian-Jiao Lyu, Ling-Yan Li, Ying Liu, Hong Zhang, Xu Wang, Xin Xi, Zong-Jun Liu, Jun-Qing Gao
• Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (Phytomedicine) Vol. 113 Pg. 154681 (May 2023) ISSN: 1618-095X [Electronic] Germany
• PMID: 36893674 (Publication Type: Journal Article)
• Copyright: Copyright © 2023. Published by Elsevier GmbH.

Chemical References

• ginsenoside Re
• MicroRNAs
• MIRN144 microRNA, rat

Topics

• Rats
• Animals
• Myocardial Reperfusion Injury (drug therapy, metabolism)
• Ferroptosis
• Myocardial Ischemia
• Reperfusion Injury (drug therapy)
• MicroRNAs (genetics, metabolism)
• Ischemia