B-Cell Receptor Features and Database Establishment in Recovered COVID-19 Patients by Combining 5'-RACE with PacBio Sequencing.

Abstract

BACKGROUND:

Antibodies induced by viral infection can not only prevent subsequent virus infection, but can also mediate pathological injury following infection. Therefore, understanding the B-cell receptor (BCR) repertoire of either specific neutralizing or pathological antibodies from patients convalescing from Coronavirus disease 2019 (COVID-19) infection is of benefit for the preparation of therapeutic or preventive antibodies, and may provide insight into the mechanisms of COVID-19 pathological injury.

METHODS:

In this study, we used a molecular approach of combining 5' Rapid Amplification of cDNA Ends (5'-RACE) with PacBio sequencing to analyze the BCR repertoire of all 5 IgH and 2 IgL genes in B-cells harvested from 35 convalescent patients after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.

RESULTS:

We observed numerous BCR clonotypes within most COVID-19 patients, but not in healthy controls, which validates the association of the disease with a prototypical immune response. In addition, many clonotypes were found to be frequently shared between different patients or different classes of antibodies.

CONCLUSIONS:

These convergent clonotypes provide a resource to identify potential therapeutic/prophylactic antibodies, or identify antibodies associated with pathological effects following infection with SARS-CoV-2.

• Authors: Zhu Zhu, Pingzhang Wang, Xiaodong Jia, Meng Yu, Huige Yan, Lei Liu, Wanbing Liu, Yaqiong Zheng, Guomei Kou, Jie Wang, Weiyan Xu, Jing Huang, Fugang Duan, Fengmin Lu, Ning Fu, Ning Zhang, Yingying Lu, Hui Dai, Shangen Zheng, Xiaoyan Qiu
• Journal: Frontiers in bioscience (Landmark edition) (Front Biosci (Landmark Ed)) Vol. 28 Issue 2 Pg. 40 (02 28 2023) ISSN: 2768-6698 [Electronic] Singapore
• PMID: 36866541 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2023 The Author(s). Published by IMR Press.

Chemical References

• Receptors, Antigen, B-Cell
• Antibodies

Topics

• Humans
• COVID-19
• SARS-CoV-2
• Receptors, Antigen, B-Cell (genetics)
• Antibodies
• B-Lymphocytes