Snakebite envenoming is a medical emergency requiring urgent and specific treatment. Unfortunately,
snakebite diagnostics are scarce, time-consuming and lacking specificity. Hence, this study aimed to develop a simple, quick and specific
snakebite diagnostic assay using animal
antibodies.
Anti-venom horse
immunoglobulin G (
IgG) and chicken
immunoglobulin Y (
IgY) were produced against the
venoms of four major medically important snake species in Southeast Asia, i.e., the Monocled Cobra (Naja kaouthia), Malayan Krait (Bungarus candidus), Malayan Pit Viper (Calloselasma rhodostoma), and White-lipped Green Pit Viper (Trimeresurus albolabris). Different capture:detection configurations of double-antibody sandwich
enzyme-linked
immunosorbent assay (ELISA) were constructed using both
immunoglobulins, and the horse
IgG:
IgG-HRP configuration was found to be most selective and sensitive in detecting the corresponding
venoms. The method was further streamlined to develop a rapid immunodetection assay, which is able to produce a visual color change within 30 min for discrimination between different snake species. The study shows it is feasible to develop a simple, quick and specific immunodiagnostic assay using horse
IgG, which can be derived directly from
antisera prepared for
antivenom production. The proof-of-concept indicates it is a sustainable and affordable approach in keeping with on-going
antivenom manufacturing activities for specific species in the region.