Anastrozole-mediated modulation of mitochondrial activity by inhibition of mitochondrial permeability transition pore opening: an initial perspective.

Abstract

The mitochondrial permeability transition pore (mtPTP) plays a vital role in altering the structure and function of mitochondria. Cyclophilin D (CypD) is a mitochondrial protein that regulates mtPTP function and a known drug target for therapeutic studies involving mitochondria. While the effect of aromatase inhibition on the mtPTP has been studied previously, the effect of anastrozole on the mtPTP has not been completely elucidated. The role of anastrozole in modulating the mtPTP was evaluated by docking, molecular dynamics and network-guided studies using human CypD data. The peripheral blood mononuclear cells (PBMCs) of patients with mitochondrial disorders and healthy controls were treated with anastrozole and evaluated for mitochondrial permeability transition pore (mtPTP) function and apoptosis using a flow cytometer. Spectrophotometry was employed for estimating total ATP levels. The anastrozole-CypD complex is more stable than cyclosporin A (CsA)-CypD. Anastrozole performed better than cyclosporine in inhibiting mtPTP. Additional effects included inducing mitochondrial membrane depolarization and a reduction in mitochondrial swelling and superoxide generation, intrinsic caspase-3 activity and cellular apoptosis, along with an increase in ATP levels. Anastrozole may serve as a potential therapeutic agent for mitochondrial disorders and ameliorate the clinical phenotype by regulating the activity of mtPTP. However, further studies are required to substantiate our preliminary findings.Communicated by Ramaswamy H. Sarma.

• Authors: Somesh Kumar, Neha Choudhary, Mohammed Faruq, Arun Kumar, Ravindra K Saran, Prem Kumar Indercanti, Vikram Singh, Haseena Sait, Sunita Jaitley, Martin Valis, Kamil Kuca, Sunil K Polipalli, Manoj Kumar, Tejveer Singh, Prashanth Suravajhala, Rohit Sharma, Seema Kapoor
• Journal: Journal of biomolecular structure & dynamics (J Biomol Struct Dyn) Vol. 41 Issue 23 Pg. 14063-14079 ( 2023) ISSN: 1538-0254 [Electronic] England
• PMID: 36815262 (Publication Type: Journal Article)

Chemical References

• Mitochondrial Permeability Transition Pore
• Anastrozole
• Mitochondrial Membrane Transport Proteins
• Peptidyl-Prolyl Isomerase F
• Cyclophilins
• Adenosine Triphosphate

Topics

• Humans
• Mitochondrial Permeability Transition Pore (metabolism, pharmacology)
• Anastrozole (pharmacology, metabolism)
• Mitochondrial Membrane Transport Proteins (metabolism, pharmacology)
• Leukocytes, Mononuclear (metabolism)
• Mitochondria (metabolism)
• Peptidyl-Prolyl Isomerase F
• Cyclophilins (genetics, metabolism)
• Adenosine Triphosphate (metabolism)
• Mitochondrial Diseases (metabolism)