Abstract | Background: Materials and Methods:
Lung cancer A549 cell cultures were stimulated for 24 h with methadone (5, 10, and 20 μM) and naloxone (20 and 40 μM) concentrations. The level of TLR4 expression was determined by the quantitative real-time polymerase chain reaction. Migration of the A549 cells was investigated after a 4-h incubation period with methadone using the Boyden Chamber assay. Results: Migration rate of the A549 cells treated with 5 (P < 0.05) and 20 (P < 0.01) μM methadone was, respectively, increased and decreased with 20 μM naloxone (P < 0.05). Furthermore, the TLR4 expression was enhanced with 5 (P < 0.05) and 20 (P < 0.01) μM methadone and decreased with 20 (P < 0.05) and 40 μM naloxone (P < 0.01). In addition, in silico docking analysis revealed docking of methadone to MD-2 and TLR4. Conclusion: According to the present DATA, methadone affects the TLR4 expression. It may however cause adverse consequences by increasing the TLR4 expression. Therefore, the useful analgesic properties of methadone should be separated from the unwanted TLR4-mediated side effects.
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Authors | Golnaz Vaseghi, Nastaran Rashidi, Nasrin Zare, Fahimeh Ghasemi, Marjan Pourhadi, Laleh Rafiee, Shaghayegh Haghjooy Javanmard |
Journal | Advanced biomedical research
(Adv Biomed Res)
Vol. 11
Pg. 122
( 2022)
ISSN: 2277-9175 [Print] India |
PMID | 36798925
(Publication Type: Journal Article)
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Copyright | Copyright: © 2022 Advanced Biomedical Research. |