Short-term flaxseed oil, rich in omega 3, protects mice against metabolic damage caused by high-fat diet, but not inflammation.

Abstract

It is known that long-term high-fat diet (HF) feeding drastically affects the adipose tissue, contributing to metabolic disorders. Recently, short-term HF consumption was shown to affect different neuronal signaling pathways. Thus, we aimed to evaluate the inflammatory effects of a short-term HF and whether a diet containing omega-3 fatty acid fats from flaxseed oil (FS) has protective effects. Mice were divided into three groups for 3 d, according to their diets: Control group (CT), HF, or FS for 3 d. Lipid profiles were assessed through mass spectrometry and inflammatory markers by RT-qPCR and Western blotting. After short-term HF, mice increased food intake, body weight, adiposity, and fasting glucose. Increased mRNA content of Ccl2 and Tnf was demonstrated in the HF compared to CT in mesenteric adipose tissue. In the liver, TNFα protein was higher in the HF group than in CT, followed by a decreased polyunsaturated fatty acids tissue incorporation in HF. On the other hand, the consumption of FS reduced food intake and fasting glucose, as well as increased omega-3 fatty acid incorporation in MAT and the liver. However, short-term FS was insufficient to control the early inflammation triggered by HF in MAT and the liver. These data demonstrated that a 3-d HF diet is enough to damage glucose homeostasis and trigger inflammation. In contrast, short-term FS protects against increased food intake and fasting glucose but not inflammation in mice.

• Authors: Susana Castelo Branco Ramos Nakandakari, Rafael Calais Gaspar, Gabriel Keine Kuga, Camila de Oliveira Ramos, Renan Fudoli Vieira, Thaiane da Silva Rios, Vitor Rosetto Muñoz, Marcella Ramos Sant'ana, Fernando Moreira Simabuco, Adelino Sanchez Ramos da Silva, Leandro Pereira Moura, Eduardo Rochete Ropelle, José Rodrigo Pauli, Dennys Esper Cintra
• Journal: The Journal of nutritional biochemistry (J Nutr Biochem) Vol. 114 Pg. 109270 (04 2023) ISSN: 1873-4847 [Electronic] United States
• PMID: 36706930 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2023 Elsevier Inc. All rights reserved.

Chemical References

• Linseed Oil
• Fatty Acids, Omega-3
• Glucose

Topics

• Mice
• Animals
• Diet, High-Fat (adverse effects)
• Linseed Oil (pharmacology)
• Fatty Acids, Omega-3 (pharmacology, metabolism)
• Inflammation (metabolism)
• Adipose Tissue (metabolism)
• Glucose (metabolism)
• Mice, Inbred C57BL