Abstract | Introduction: Methods: We used GEO analysis to identify the potential RING finger protein family member that contributes to Bap-induced NSCLC. We next used RT-qPCR, Western blot and ChIP assay to investigate the potential mechanism of Bap inhibits RNF182. BGS analyses were used to analyze the methylation level of RNF182. Results: Here we reported that the carcinogen Bap suppresses the expression of ring finger protein 182 (RNF182) in non-small cell lung cancer (NSCLC) cells, which is mediated by abnormal hypermethylation in an AhR independent way and transcriptional regulation in an AhR dependent way. Furthermore, RNF182 exhibits low expression and hypermethylation in tumor tissues. RNF182 also significantly suppresses cell proliferation and induces cell cycle arrest in NSCLC cell lines. Conclusion: These results demonstrated that Bap inhibits RNF182 expression to promote lung cancer tumorigenesis through activating AhR and promoting abnormal methylation.
|
Authors | Yating Liu, Lianlian Ouyang, Chao Mao, Yuanbing Chen, Na Liu, Ling Chen, Ying Shi, Desheng Xiao, Shuang Liu, Yongguang Tao |
Journal | Frontiers in oncology
(Front Oncol)
Vol. 12
Pg. 1009508
( 2022)
ISSN: 2234-943X [Print] Switzerland |
PMID | 36686776
(Publication Type: Journal Article)
|
Copyright | Copyright © 2023 Liu, Ouyang, Mao, Chen, Liu, Chen, Shi, Xiao, Liu and Tao. |