Abstract | BACKGROUND: METHODS:
Neuropathic pain was induced by repeated administration of recombinant gp120 with morphine (gp120/M) in rats. Mechanical allodynia was assessed using von Frey filaments, and thermal latency using hotplate test. Protein expression of spinal TNFRI and cleaved caspase-11 was examined using western blots. The image of spinal mitochondrial superoxide was examined using MitoSox Red (mitochondrial superoxide indicator) image assay. Immunohistochemistry was used to examine the location of TNFRI and caspase-11 in the SCDH. Intrathecal administration of antisense oligodeoxynucleotide (AS-ODN) against TNFRI, caspase-11 siRNA, or a scavenger of mitochondrial superoxide was given for antinociceptive effects. Statistical tests were done using analysis of variance (1- or 2-way), or 2-tailed t test. RESULTS: CONCLUSIONS: These results suggest that spinal TNFRI-mtROS- caspase 11 signal pathway plays a critical role in the HIV-associated neuropathic pain state, providing a novel approach to treating chronic pain in PLWH with opioids.
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Authors | Kentaro Hayashi, Hyun Yi, Xun Zhu, Shue Liu, Jun Gu, Keiya Takahashi, Yuta Kashiwagi, Marta Pardo, Hirotsugu Kanda, Heng Li, Roy C Levitt, Shuanglin Hao |
Journal | Anesthesia and analgesia
(Anesth Analg)
Vol. 136
Issue 4
Pg. 789-801
(04 01 2023)
ISSN: 1526-7598 [Electronic] United States |
PMID | 36662639
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Copyright | Copyright © 2023 International Anesthesia Research Society. |
Chemical References |
- Reactive Oxygen Species
- tempol
- Superoxides
- Morphine
- Tumor Necrosis Factor-alpha
- RNA, Small Interfering
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Topics |
- Rats
- Humans
- Animals
- Reactive Oxygen Species
(metabolism)
- Hyperalgesia
(metabolism)
- Superoxides
(metabolism)
- Morphine
(adverse effects)
- Chronic Pain
(metabolism)
- Rats, Sprague-Dawley
- HIV Infections
(metabolism, pathology)
- Neuralgia
(metabolism)
- Tumor Necrosis Factor-alpha
(metabolism)
- RNA, Small Interfering
(adverse effects, metabolism)
- Spinal Cord
(metabolism)
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