Andrographolide-treated bone marrow mesenchymal stem cells-derived conditioned medium protects cardiomyocytes from injury by metabolic remodeling.

Abstract

BACKGROUND:

Bone marrow mesenchymal stem cells (BMSCs) transplantation therapy providing a great hope for the recovery of myocardial ischemic hypoxic injury. However, the microenvironment after myocardial injury is not conducive to the survival of BMSCs, which limits the therapeutic application of BMSCs. Our previous study has confirmed that the survival of BMSCs cells in the glucose and serum deprivation under hypoxia (GSDH) is increased after Andrographolide (AG) pretreatment, but whether this treatment could improve the effect of BMSCs in repairing of myocardial injury has not been verified.

METHODS AND RESULT:

We first treated H9C2 with GSDH to simulate the microenvironment of myocardial injury in vitro, then we pretreated rat primary BMSCs with AG, and collected conditioned medium derived from BMSCs (BMSCs-CM) and conditioned medium derived from AG-pretreated BMSCs (AG-BMSCs-CM) after GSDH treatment. And they were used to treat H9C2 cells under GSDH to further detect oxidative stress and metabolic changes. The results showed that AG-BMSCs-CM could be more advantageous for cardiomyocyte injury repair than BMSCs-CM, as indicated by the decrease of apoptosis rate and oxidative stress. The changes of mitochondria and lipid droplets results suggested that AG-BMSCs-CM can regulate metabolic remodeling of H9C2 cells to repair cell injury, and that AMPK was activated during this process.

CONCLUSIONS:

This study demonstrates, for the first time, the protective effect of AG-BMSCs-CM on GSDH-induced myocardial cell injury, providing a potential therapeutic strategy for clinical application.

• Authors: Yanting Sun, Hao Xu, Bin Tan, Qin Yi, Huiwen Liu, Jie Tian, Jing Zhu
• Journal: Molecular biology reports (Mol Biol Rep) Vol. 50 Issue 3 Pg. 2651-2662 (Mar 2023) ISSN: 1573-4978 [Electronic] Netherlands
• PMID: 36641493 (Publication Type: Journal Article)
• Copyright: © 2023. The Author(s), under exclusive licence to Springer Nature B.V.

Chemical References

• Culture Media, Conditioned
• andrographolide

Topics

• Rats
• Animals
• Myocytes, Cardiac (metabolism)
• Culture Media, Conditioned (pharmacology, metabolism)
• Rats, Sprague-Dawley
• Mesenchymal Stem Cells (metabolism)
• Apoptosis
• Mesenchymal Stem Cell Transplantation (methods)
• Bone Marrow Cells (metabolism)