Periodontitis is an inflammatory disease induced by plaque microorganisms. In the clinic,
antibiotic assistant periodontal mechanical
therapy is the most effective
therapy for the treatment of
periodontitis. However, the drug resistance of the
antibiotics and the repeated coming and diminishing of the disorder of oxidation-reduction balance in the inflammatory tissue could not meet the high requirements for
periodontic health control in long periods.
Deuterohemin-ala-his-thr-val-glu-lys (DhHP-6) is a biomimetic
oxidase-mimicking
enzyme that simulates the reactive
oxygen radical scavenger function of
heme by synthesizing the new molecular material following the key structure and amino acid sequence of
heme. In this article, we report the
antioxidant and anti-inflammatory properties of
DhHP-6 by building a inflammatory model for human gingival fibroblasts (HGFs) stimulated by lipolysaccharide (LPS) and its effects on
periodontitis in Wistar rats.
DhHP-6 reduced the oxidative stress of HGFs by increasing the amount of the
reductase species of
glutathione (GSH) and
catalase (CAT) while decreasing the amount of
oxidase species of
malonaldehyde (MDA) and
reactive oxygen species (ROS).
DhHP-6 had a dose-dependent protective effect on alveolar bone absorption in rats with
periodontitis, enhanced
antioxidant capacity, and reduced
inflammation. As determined by Micro-CT scanning,
DhHP-6 reduced
alveolar bone loss and improved the bone structure of the left maxillary first molar of rats. There were no obvious morphological and histological differences in the rat organs with or without
DhHP-6 treatment. These results suggest that
DhHP-6 can be used to treat
periodontitis by increasing the expression levels of
antioxidant enzymes and
antioxidants in systemic and local tissues, thereby reducing levels of oxidation products and cyto-inflammatory factors. The synergistic
antioxidant and anti-inflammatory effects of
DhHP-6 suggest that there are promising applications of this biomimetic
enzyme molecular material for the next generation of agents for
periodontitis therapy.