Abstract | BACKGROUND: METHODS: Confocal laser scanning microscopy was used to evaluate the uptake of nanoparticles (NPs). The potential functions of USP30 were evaluated by cell counting kit (CCK)-8, flow cytometry, biochemical assay, coimmunoprecipitation, qRT-PCR, and immunoblotting. The antitumor effect of NP-loaded USP30 inhibitor MF-094 was evaluated in vitro and in vivo. RESULTS: In this study, increased USP30 expression was found in OSCC specimens and cell lines through qRT-PCR and immunoblotting. CCK-8, flow cytometry, and biochemical assay revealed that the deubiquitylated catalytic activity of USP30 contributed to cell viability and glutamine consumption of OSCC. Subsequently, USP30 inhibitor MF-094 was loaded in ZIF-8-PDA and PEGTK to fabricate ZIF-8-PDA-PEGTK nanoparticles, which exhibited excellent inhibition of cell viability and glutamine consumption of OSCC, both in vitro and in vivo. CONCLUSION: The results indicated the clinical significance of USP30 and showed that nanocomposites provide a targeted drug delivery system for treating OSCC.
|
Authors | Xinyu Zhang, Yong Han, Shuli Liu, Bing Guo, Shengming Xu, Yue He, Liu Liu |
Journal | Cellular & molecular biology letters
(Cell Mol Biol Lett)
Vol. 27
Issue 1
Pg. 107
(Dec 06 2022)
ISSN: 1689-1392 [Electronic] England |
PMID | 36474192
(Publication Type: Journal Article)
|
Copyright | © 2022. The Author(s). |
Chemical References |
- MF-094
- Glutamine
- Usp30 protein, human
- Thiolester Hydrolases
- Mitochondrial Proteins
|
Topics |
- Humans
- Mouth Neoplasms
(drug therapy)
- Squamous Cell Carcinoma of Head and Neck
(drug therapy)
- Carcinoma, Squamous Cell
(drug therapy)
- Glutamine
- Head and Neck Neoplasms
- Thiolester Hydrolases
- Mitochondrial Proteins
|