Induction of vascular endothelial growth factor-A165a in human retinal and endothelial cells in response to glyoxal.

Abstract

Low-density lipoprotein (LDL) apheresis is effective and safe for patients with diabetes, proteinuria, and dyslipidemia. Diabetes mellitus is accompanied by ocular microvascular complications like retinal neovascularization or diabetic macular edema. These are leading causes of blindness and can be mediated by abnormal vessel growth and increased vascular permeability due to elevated levels of vascular endothelial growth factor (VEGF) in diabetic patients. In this study, we established methods to study the expression of different VEGF isoforms in human retinal and endothelial cells. The VEGF-A165a isoform is much higher expressed in retinal cells, compared to endothelial cells. Stimulation with glyoxal as a model of oxidative stress under diabetic conditions lead to a pronounced induction of VEGF-A165a in human retinal and endothelial cells. These data suggest that diabetes and oxidative stress induce VEGF-A isoforms which could be relevant in regulating the ingrowths of novel blood vessels into the retina in diabetic patients.

• Authors: Henning Morawietz, Annika Frenzel, Alice Mieting, Winfried Goettsch, Monika Valtink, Cora Roehlecke, József Jászai, Richard H W Funk, Klio A Becker, Katrin Engelmann
• Journal: Therapeutic apheresis and dialysis : official peer-reviewed journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy (Ther Apher Dial) Vol. 26 Suppl 1 Pg. 29-34 (Dec 2022) ISSN: 1744-9987 [Electronic] Australia
• PMID: 36468302 (Publication Type: Journal Article)
• Copyright: © 2022 The Authors. Therapeutic Apheresis and Dialysis published by John Wiley & Sons Australia, Ltd on behalf of International Society for Apheresis and Japanese Society for Apheresis.

Chemical References

• Vascular Endothelial Growth Factor A
• Glyoxal
• Protein Isoforms

Topics

• Humans
• Vascular Endothelial Growth Factor A (metabolism)
• Diabetic Retinopathy (therapy, etiology, metabolism)
• Endothelial Cells (metabolism)
• Glyoxal (pharmacology, metabolism)
• Macular Edema
• Retina (metabolism)
• Protein Isoforms (metabolism)