Abstract | BACKGROUND:
Cyanidin has a protective effect on the nervous system and has been reported to treat tumor effectively. However, its impact on glioma stem cells (GSC) is unknown. METHODS: RESULTS:
Cyanidin significantly reduces the cell viability of all GSCs, and exhibited the most substantial effect in GBM2 but no apparent effect in 293T cells. It can regulate the Wnt signaling pathway of all GSC lines. In the GBM2, GBM7, G166, and G179 cell lines, there is upregulation of WNT1 and MYC genes, while in the G144 and GliNS2 cell line, these two genes are down-regulated after cyanidin treatment. Cytoplasmic and nuclear protein levels of β- catenin in all cell lines are down-regulated. Cyanidin treatment significantly decreases the protein level for c-Myc in the GBM2 cell line compared with untreated cells, not in G144 or GliNS2 cells. Furthermore, cyanidin strongly reduces the expression of Twist1 and Snail1 in GBM2, G179, and G144 cell lines, while the GliNS2 cells show an opposite change in the cytoplasm and no change in nuclear. CONCLUSION:
Cyanidin exerts an anti- tumor effect in glioma stem cell lines, probably through the Wnt signaling pathway.
|
Authors | Zicheng Xue, Lei Tian, Hui Zheng, Yucai Zhang, Junying Song |
Journal | The International journal of neuroscience
(Int J Neurosci)
Pg. 1-8
(Dec 11 2022)
ISSN: 1563-5279 [Electronic] England |
PMID | 36458565
(Publication Type: Journal Article)
|