Abstract |
The clinical utility of doxorubicin is compromised due to dose related toxic side effects and limited oral bioavailability with no oral formulation being marketed. Enhancement of intestinal absorption and magnification of cytotoxicity can overcome these limitations. Accordingly, the objective was to probe penetration enhancers, bilosomes and their combinations for enhanced intestinal absorption and improved cytotoxicity of doxorubicin. Piperine and dipyridamole were tested as enhancers alone or encapsulated in bilosomes comprising Span60, cholesterol and bile salts. Bilosomes were nanosized spherical vesicles with negative zeta potential and were able to entrap doxorubicin with efficiency ranging from 45.3 % to 53 %. Intestinal absorption studies utilized in-situ rabbit intestinal perfusion which revealed site dependent doxorubicin absorption correlating with regional distribution of efflux transporters. Co-perfusion with the enhancer increased intestinal absorption with further augmentation after bilosomal encapsulation. The latter increased the % fraction absorbed by 4.5-6 and 1.8-2.5-fold from jejuno-ileum and colon, respectively, depending on bilosomes composition. Additionally, doxorubicin cytotoxicity against breast cancer cells (MCF-7) was significantly improved after bilosomal encapsulation and the recorded doxorubicin IC50 value was reduced from 13.3 μM to 0.1 μM for the best formulation. The study introduced bilosomes encapsulating absorption enhancers as promising carriers for enhanced cytotoxicity and oral absorption of doxorubicin.
|
Authors | Amal A Sultan, Ghada A Saad, Gamal M El Maghraby |
Journal | International journal of pharmaceutics
(Int J Pharm)
Vol. 630
Pg. 122427
(Jan 05 2023)
ISSN: 1873-3476 [Electronic] Netherlands |
PMID | 36435504
(Publication Type: Journal Article)
|
Copyright | Copyright © 2022 Elsevier B.V. All rights reserved. |
Chemical References |
- Liposomes
- Bile Acids and Salts
- Doxorubicin
|
Topics |
- Animals
- Rabbits
- Liposomes
- Administration, Oral
- Intestinal Absorption
- Bile Acids and Salts
- Doxorubicin
(pharmacology)
|