Protective Effect of Resveratrol in an Experimental Model of Salicylate-Induced Tinnitus.

Abstract

To date, the effect of resveratrol on tinnitus has not been reported. The attenuative effects of resveratrol (RSV) on a salicylate-induced tinnitus model were evaluated by in vitro and in vivo experiments. The gene expression of the activity-regulated cytoskeleton-associated protein (ARC), tumor necrosis factor-alpha (TNFα), and NMDA receptor subunit 2B (NR2B) in SH-SY5Y cells was examined using qPCR. Phosphorylated cAMP response element-binding protein (p-CREB), apoptosis markers, and reactive oxygen species (ROS) were evaluated by in vitro experiments. The in vivo experiment evaluated the gap-prepulse inhibition of the acoustic startle reflex (GPIAS) and auditory brainstem response (ABR) level. The NR2B expression in the auditory cortex (AC) was determined by immunohistochemistry. RSV significantly reduced the salicylate-induced expression of NR2B, ARC, and TNFα in neuronal cells; the GPIAS and ABR thresholds altered by salicylate in rats were recovered close to their normal range. RSV also reduced the salicylate-induced NR2B overexpression of the AC. These results confirmed that resveratrol exerted an attenuative effect on salicylate-induced tinnitus and may have a therapeutic potential.

• Authors: Anji Song, Gwang-Won Cho, Changjong Moon, Ilyong Park, Chul Ho Jang
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 23 Issue 22 (Nov 16 2022) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 36430660 (Publication Type: Journal Article)

Chemical References

• Resveratrol
• Salicylates
• Tumor Necrosis Factor-alpha

Topics

• Animals
• Humans
• Rats
• Neuroblastoma
• Rats, Sprague-Dawley
• Resveratrol (pharmacology, therapeutic use)
• Salicylates (pharmacology)
• Tinnitus (chemically induced, drug therapy, pathology)
• Tumor Necrosis Factor-alpha (therapeutic use)
• Disease Models, Animal