Alzheimer's disease (AD), a relentless
neurodegenerative disorder, is still waiting for safer profile drugs, risk factors affecting AD's pathogenesis include aβ accumulation,
tau protein hyperphosphorylation, and
neuroinflammation. This research aimed to synthesize 2-amino-6‑trifluoromethoxy
benzothiazole schiff bases. Synthesis was straightforward, combining the
riluzole skeleton with compounds containing the
azomethine group.
Schiff bases synthesized were characterized spectroscopically using
proton NMR (1H NMR), and FTIR. In-vivo biological evaluation against
scopolamine-induced neuronal damage revealed that these newly synthesized
schiff bases were effective in protecting neurons against neuroinflammatory mediators. In-vitro results revealed that these compounds had remarkable potential in improving the
anti-oxidant levels. It downregulated
glutathione (GSH),
glutathione S-transferase (GST),
catalase levels, and upregulated lipid peroxidation (LPO) levels. Immunohistochemical studies revealed that groups treated with the newly synthesized
schiff bases had reduced expression of inflammatory mediators such as
cyclooxygenase 2 (COX-2), JNK,
tumor necrosis factor (TNF-α),
nuclear factor kappa B (
NF-kB) in contrast to the disease group. Moreover, molecular docking studies on these compounds also showed that they possessed a better binding affinity for above mentioned inflammatory mediators. The results of these studies showed that 2-amino-6-trifluoromethoxy
benzothiazole schiff bases are remarkably effective against oxidative stress-mediated
neuroinflammation.