Are intratumoral microbiota involved in the progression of intraductal papillary mucinous neoplasms of the pancreas?

Abstract

BACKGROUND:

Microbiota have been reported to influence the development of various gastrointestinal neoplasms through the mechanism of sustained inflammation; however, few data are available regarding their influence on intraductal papillary mucinous neoplasms. The aim of this study was to assess the association between specific microbiota and the clinicopathologic characteristics of intraductal papillary mucinous neoplasms of the pancreas.

METHODS:

DNA was extracted from formalin-fixed, paraffin-embedded samples of 30 patients who underwent pancreatectomy for intraductal papillary mucinous neoplasm, and polymerase chain reaction was used to create sequence libraries using the primer set for the V3 and V4 region of 16S recombinant DNA. Filtered sequence reads were then processed into operational taxonomic units with a 97% identity threshold and the relative abundance of bacteria compared between the 2 groups using operational taxonomic units.

RESULTS:

There was a trend toward fewer Firmicutes and more Proteobacteria and Fusobacteria in the relative abundance of main duct operational taxonomic units than in branch duct operational taxonomic units. The relative abundances of Bacteroidetes (P < .01) and Fusobacteria (P = .04) were significantly higher in invasive intraductal papillary mucinous neoplasms than in noninvasive intraductal papillary mucinous neoplasms. The relative abundance of the intestinal type was significantly lower in Firmicutes than the relative abundance of the nonintestinal type (P = .04). Notably, main duct operational taxonomic units with the intestinal subtype were affected by increased proportions of Proteobacteria and Fusobacteria, and Fusobacteria were abundant in the intestinal type of invasive main duct operational taxonomic units.

CONCLUSION:

Intratumoral microbiota may be involved in the progression of operational taxonomic units.

• Authors: Yuto Hozaka, Hideyuki Oi, Souichi Satake, Yoshinori Uchino, Yuichi Goto, Tetsuya Idichi, Kiyonori Tanoue, Yoichi Yamasaki, Yota Kawasaki, Yuko Mataki, Hiroshi Kurahara, Akihiro Nakajo, Michiyo Higashi, Akihide Tanimoto, Tsuyoshi Sugiura, Takao Ohtsuka
• Journal: Surgery (Surgery) Vol. 173 Issue 2 Pg. 503-510 (02 2023) ISSN: 1532-7361 [Electronic] United States
• PMID: 36404180 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2022 Elsevier Inc. All rights reserved.

Topics

• Humans
• Carcinoma, Pancreatic Ductal (pathology)
• Pancreatic Intraductal Neoplasms (surgery)
• Adenocarcinoma, Mucinous (surgery, pathology)
• Pancreatic Neoplasms (pathology)
• Pancreas (surgery)
• Pancreatectomy