In Vivo Assessments of Mesoblastic Nephroma (Ne/De) and Myelomonoblastic Leukaemia (My1/De) Tumour Development in Hypercholesterolemia Rat Models.

Abstract

Given the rising prevalence of lipid metabolic disorders and malignant diseases, we aimed to establish an in vivo hypercholesterinaemic tumour-bearing rat model for the induction and assessment of these conditions. A normal standard CRLT/N, 2 (baseline),- or 4 (2 + 2, pretreated)-week-long butter and cholesterol rich (BCR) diet was applied to mesoblastic nephroma (Ne/De) and myelomonoblastic leukaemia (My1/De) tumour-bearing and healthy control Long—Evans and Fischer 344 rats. The beginning of chow administration started in parallel with tumour induction and the 2 weeks of pre-transplantation in the baseline and pretreated groups, respectively. Fourteen days post-inoculation, the measurement of lipid parameters and [18F]F-FDG PET/MRI examinations was executed. The comparable lipid status of baseline healthy and tumorous rats proves that regardless of tumour presence, BCR-based hypercholesterolemia was achieved. A higher tumour mass among pretreated tumorous animals was found when compared to the control groups (p < 0.05, p < 0.01). Further, a visually greater [18F]F-FDG accumulation was observed in pretreated BCR tumorous animals; however, the quantitative data (SUVmean: 9.86 ± 0.98, 9.68 ± 1.24; SUVmax: 19.63 ± 1.20; 17.56 ± 3.21 for Ne/De and My1/De, respectively) were not statistically significantly different from those of the CRLT/N tumorous rats (SUVmean: 8.40 ± 1.42, 7.22 ± 1.06 and SUVmax: 15.99 ± 2.22, 12.46 ± 1.96 for control Ne/De and My1/De, respectively). Our model seems to be appropriate for simultaneously investigating hypercholesterolemia and cancer in the same rat.

• Authors: Zita Képes, Alexandra Barkóczi, Judit P Szabó, Ibolya Kálmán-Szabó, Viktória Arató, Ildikó Garai, Péter Árkosy, István Jószai, Ádám Deák, István Kertész, István Hajdu, György Trencsényi
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 23 Issue 21 (Oct 27 2022) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 36361850 (Publication Type: Journal Article)

Chemical References

• Fluorodeoxyglucose F18
• Lipids
• Radiopharmaceuticals

Topics

• Animals
• Rats
• Fluorodeoxyglucose F18
• Nephroma, Mesoblastic
• Hypercholesterolemia
• Rats, Long-Evans
• Positron-Emission Tomography
• Kidney Neoplasms (diagnostic imaging)
• Leukemia
• Lipids
• Radiopharmaceuticals
• Positron Emission Tomography Computed Tomography